Nicotiana nucleic acid molecules and uses thereof
AssigneeU.S. Smokless Tobacco Company
InventorsDongmei Xu, Mark T. Nielsen
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Abstract
The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.
Claims12
A method for reducing the expression of nicotine demethylase in a tobacco plant, said method comprising expressing a transgene encoding a double-stranded RNA molecule that inhibits expression of said nicotine demethylase in said plant, said double-stranded RNA molecule comprising at least 25 consecutive nucleotides that have 91% or greater sequence identity to an exon of SEQ ID NO:
- 4, wherein expression of said nicotine demethylase is inhibited in said plant relative to a tobacco plant lacking said transgene.
Dependencies are inferred from the claim text.