Metal resistant plants and phytoremediation of environmental contamination
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsRichard B. Meagher, Yujing Li, Om P. Dhankher
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Abstract
The present disclosure provides a method of producing transgenic plants which are resistant to at least one metal ion by transforming the plant with a recombinant DNA comprising a nucleic acid encoding a bacterial arsenic reductase under the control of a plant expressible promoter, and a nucleic acid encoding a nucleotide sequence encoding a phytochelatin biosynthetic enzyme under the control of a plant expressible promoter. The invention also relates a method of phytoremediation of a contaminated site by growing in the site a transgenic plant expressing a nucleic acid encoding a bacterial arsenate reductase and a nucleic acid encoding a phytochelatin biosynthetic enzyme.
Claims16
A method for producing a transgenic plant which is resistant to at least one metal ion, said method comprising the steps of:
- (a) introducing a nucleic acid molecule comprising a bacterial arsenate reductase coding sequence operably linked to a plant expressible transcription regulatory sequence, into a plant cell or into plant tissue to produce a transformed plant cell or transformed plant tissue;
- (b) introducing at least one plant expressible phytochelatin biosynthetic enzyme coding sequence into the transgenic plant cell or transgenic tissue, wherein said phytochelatin biosynthetic enzyme is selected from the group consisting of gamma-glutamyl-cysteine synthase, phytochelatin synthase or glutathione synthase;
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- (c) regenerating a transgenic plant from the transgenic plant cell or transgenic plant tissue, whereby a metal ion resistant plant is produced.
Dependencies are inferred from the claim text.