Composition for culturing mouse pluripotent stem cells
AssigneeRiken
InventorsHitoshi Niwa, Kazuya Ogawa
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Abstract
The invention aims to proliferate or establish undifferentiated pluripotent stem cells that retain their differentiation potency by culturing pluripotent stem cells in a medium free of a feeder cell, or a serum. The aim is attained by using a culture medium for pluripotent stem cells comprising the known ingredients, which is supplemented with an inhibitor of an adenylate cyclase activity.
Claims15
A process for culturing mouse pluripotent stem cells, which comprises culturing the mouse pluripotent stem cells in a medium comprising leukemia inhibitory factor (LIF), an antioxidant and an inhibitor of adenylate cyclase activity, said process allowing the mouse pluripotent stem cells to proliferate or establish while maintaining the cells in an undifferentiated state.
A process for the preparation of a clonal population of undifferentiated mouse pluripotent stem cells, which comprises culturing the undifferentiated mouse pluripotent stem cells in a medium comprising leukemia inhibitory factor (LIF), an antioxidant and an inhibitor of adenylate cyclase activity.
A process for the preparation of a clonal population of undifferentiated mouse pluripotent stem cells, which comprises isolating undifferentiated pluripotent stem cells from a living body of a mouse, and culturing the undifferentiated mouse pluripotent stem cells in a medium comprising leukemia inhibitory factor (LIF), an antioxidant and an inhibitor of adenylate cyclase activity.
Dependencies are inferred from the claim text.