# US 7,700,350 B2 · Recombinant vector containing infectious human cytomegalovirus genome with preserved wild-type characteristics of clinical isolates

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- Number: US 7,700,350 B2
- Document ID: US7700350B2
- Status: Granted
- Kind: B2
- Application number: 11/180,000
- Filed: 2005-07-13
- Published: 2006-01-26
- Granted: 2010-04-20
- Earliest claimed: 2001-02-21
- Assignees: Vical Incorporated
- Inventors: Gabriele Hahn
- Web page: https://patentgrep.com/patent/US7700350B2

## CPC

- C12N 15/86: Viral vectors
- A61K 39/12: Viral antigens
- A61K 39/245: Herpetoviridae, e.g. herpes simplex virus
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61K 48/00: Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C12N 2710/16132: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2710/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2710/16143: viral genome or elements thereof as genetic vector
- C12N 2710/16161: Methods of inactivation or attenuation
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A recombinant vector containing infectious genome of human cytomegalovirus (HCMV) and being useful for the production of reconstituted HCMV virus retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells and to induce microfusion is characterized in that it is obtainable by inserting DNA from a clinical isolate of HCMV virus into a bacterial cloning vehicle. Such vector can be used e.g., for production of reconstituted HCMV virus retaining the phenotypic characteristics of a parental clinical isolate and for studying genes and functions of genes of HCMV virus. A further aspect are mutant viruses and inter alia their use for studying aspects of infectivity of HCMV virus.

## Claims

### Claim 1

A recombinant vector containing the infectious genome of human cytomegalovirus (HCMV) retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells, to induce microfusion and to spread viral material from human umbilical vein endothelial cells (HUVEC) to polymorphonuclear leukocytes (PMNL), obtainable by inserting DNA from a clinical isolate of HCMV virus into a bacterial cloning vehicle wherein the recombinant vector is FIX-Bac-7.

### Claim 2

A bacterial cell line containing a recombinant vector containing an infectious genome of human cytomegalovirus (HCMV) retaining phenotypic characteristics of a clinical virus isolate including the ability to grow on endothelial cells, to induce microfusion and to spread viral material from human umbilical vein endothelial cells (HUVEC) to polymorphonuclear leukocytes (PMNL), obtainable by inserting DNA from a clinical isolate of HCMV virus into a bacterial cloning vehicle;
wherein said bacterial cell line is designated FIX-Bac-7-E.coli DH 10B and deposited as DSM 13958 containing at least one copy of FIX-Bac-7.