Methods and kits for the in vitro diagnostic or for the monitoring of a disease involving an inflammatory reaction
OwnerXavier Forcevilleinventor
Life of this patent
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Abstract
Method for the in vitro diagnostic or for the monitoring of a disease involving an inflammatory reaction within a patient, which comprises the steps of: a) providing a biological sample from the patient; b) measuring the amount of selenoprotein P which is contained in the biological sample; c) comparing the amount of selenoprotein P measured at step b) i) with the amount of selenoprotein P which is contained in a biological sample from an individual which is not affected with the disease; or ii) with the amount of selenoprotein P which was contained in a biological sample from the same patient.
Claims5
A method for diagnosing severe Systemic Inflammation Response Syndrome (severe SIRS) or septic shock involving an inflammatory reaction within a patient, wherein said method comprises the steps of:
- (a) providing a blood sample from said patient;
- (b) providing a plurality of control samples wherein each control sample contains a predetermined concentration of selenoprotein P as a control reference;
- (c) determining the concentration of selenoprotein P which is contained in the blood sample by measuring the amount of the selenoprotein P in the blood sample, and measuring the amount of the selenoprotein P in the control samples, and then comparing the measured amount in the blood sample to the measured amount in the control samples to determine the concentration of selenoprotein P in the blood sample, wherein the determination of the selenoprotein concentration is performed by an immunoassay using antibodies directed against selenoprotein P;
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- (d) diagnosing that said patient does not have severe SIRS or septic shock when the selenoprotein P plasma concentration which is determined at step (c) is equal to or higher than 40 nmol/L, and diagnosing that said patient has severe SIRS or septic shock when the selenoprotein P plasma concentration which is determined at step (c) is equal to or lower than 30 nmol/L.
A method for monitoring severe Systemic Inflammation Response Syndrome (severe SIRS) or septic shock involving an inflammatory reaction within a patient, wherein said method comprises the steps of:
- (a) taking a blood sample from said patient, respectively i) prior to said patient being monitored and ii) during the time period wherein said patient is being monitored;
- (b) providing a plurality of control samples wherein each control sample contains a predetermined concentration of selenoprotein P as a control reference;
- (c) determining the concentration of selenoprotein P which is contained in the blood samples obtained at step a) by measuring the amount of selenoprotein P which is contained in the blood samples obtained at step a), and measuring the amount of the selenoprotein P in the control samples, and then comparing the measured amount in the blood samples to the measured amount in the control samples to determine the concentration of selenoprotein P in the blood samples, wherein the determination of the selenoprotein P concentration is performed by an immunoassay using antibodies directed against selenoprotein P;
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- (d) comparing the concentration of selenoprotein P determined at step (c) for the samples taken i) prior to said patient being monitored and ii) during the time period wherein said patient is being monitored;
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- (e) measuring an increase or a decrease of said selenoprotein P concentration in step (d), whereby said increase or decrease assists in monitoring severe SIRS or septic shock involving an inflammatory reaction.
Dependencies are inferred from the claim text.