Methods of producing pancreatic hormones
AssigneeCythera, Inc.
InventorsLaura Martinson, Evert Kroon, Kevin D'Amour, Emmanuel Edward Baetge
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Abstract
Disclosed herein are methods of producing pancreatic hormone-expressing cells by first differentiating pluripotent cells in cell culture so as to produce endodermal cells, the endodermal cells being competent to further differentiate into hormone-expressing cells capable of secreting at least one pancreatic hormone in response to a physiological signal, and then, transplanting the cultured endodermal cells into an organism, such as an organism in need of an endocrine cell therapy.
Claims15
A method for producing insulin, said method comprising the steps of:
- (a) contacting human embryonic stem (hES) cells in vitro with a first medium comprising an agent that activates a TGF-β receptor family member;
- (b) culturing in vitro the hES-derived cells of step (a) in a second medium lacking a substantial amount of the agent that activates the TGF-β receptor family member, thereby generating PDX-1 positive pancreatic endoderm cells;
- (c) transplanting the PDX-1 positive pancreatic endoderm cells of step (b) into a mammalian subject;
- and
- (d) maturing the PDX-1 positive pancreatic endoderm cells of step (c) in vivo, thereby obtaining insulin secreting cells, wherein the insulin secreting cells secrete insulin in response to glucose stimulation.
A method for producing insulin, said method comprising the steps of:
- (a) contacting human embryonic stem (hES) cells in vitro with a medium comprising a first agent that activates a TGF-β receptor family member;
- (b) culturing in vitro the hES-derived cells of step (a) in a second medium comprising a second agent that inhibits the TGF-β receptor family member, thereby generating PDX-1 positive pancreatic endoderm cells;
- (c) transplanting the PDX-1 positive pancreatic endoderm cells of step (b) into a mammalian subject;
- and
- (d) maturing the PDX-1 positive pancreatic endoderm cells of step (c) in vivo, thereby obtaining insulin secreting cells, wherein the insulin secreting cells secrete insulin in response to glucose stimulation.
Dependencies are inferred from the claim text.