Methods for increasing definitive endoderm production
AssigneeCythera, Inc.
InventorsAlan Agulnick, Kevin D'Amour, Emmanuel Edward Baetge
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Abstract
Disclosed herein are methods for increasing the production of definitive endoderm cells from pluripotent stem cells. Also disclosed herein are agents capable of increasing definitive endoderm cell production.
Claims20
An in vitro composition comprising a TGFβ superfamily growth factor and an antagonist of E-cadherin, said antagonist of E-cadherin bound to E-cadherin-expressing human embryonic stem cells, wherein the binding of the antagonist inhibits adhesion between said embryonic stem cells.
A cell culture comprising a TGFβ superfamily growth factor, a calcium-binding agent and E-cadherin-expressing human embryonic stem cells in a culture medium, wherein the calcium-binding agent is bound to calcium ions in the culture medium, thereby inhibiting adhesion between said embryonic stem cells.
Dependencies are inferred from the claim text.