Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions
AssigneeBavarian Nordic A/S
InventorsIngmar Rathe, Eva Felder, Karl Heller
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Abstract
The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.
Claims20
A method for the amplification of a virus comprising:
- cultivating chicken embryo fibroblast (CEF) cells permissive for productive replication of the virus in a serum free medium comprising 1-50 ng/ml epidermal growth factor (EGF),
- infecting the CEF cells with the virus,
- cultivating the infected CEF cells in serum free medium until progeny virus is produced, and
- isolating the virus from the culture.
Dependencies are inferred from the claim text.