Cupredoxin derived transport agents and methods of use thereof
AssigneeThe Board of Trustees of the University of Illinois
InventorsAnanda Chakrabarty, Tapas Das Gupta, Tohru Yamada, Arsenio Fialho
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Abstract
The present invention discloses methods and materials for delivering a cargo compound into a cancer cell. Delivery of the cargo compound is accomplished by the use of protein transduction domains derived from cupredoxins. The invention further discloses methods for treating cancer and diagnosing cancer.
Claims32
An isolated peptide, consisting of a sequence that is a truncation of a full-length wild-type cupredoxin or H.8 outer membrane protein, and which facilitates the entry of a linked molecule into a mammalian cancer cell.
An isolated peptide which has structural homology to SEQ ID NO:
- 9 and which facilitates the entry of a linked molecule into a mammalian cancer cell through a cupredoxin entry domain or variant thereof wherein said structural homology is defined from a method selected from the group consisting of (i) calculating a VAST p value from a structural comparison between said isolated peptide and SEQ ID NO:
- 9 wherein said VAST p value is selected from the group consisting of less than about 103, less than about 105, and less than about 107 and (ii) using the DALI algorithm to compare said isolated peptide and SEQ ID NO:
- 9 and obtaining a DALI Z score selected from the group consisting of about 3.5, about 7.0, and about 10.0.
A complex comprising a cargo compound and a peptide, wherein the peptide consists of a sequence that is a truncation of a full-length wild-type cupredoxin, wherein the peptide, is linked to the cargo compound, and wherein the peptide facilitates entry of the cargo compound into a mammalian cancer cell.
An isolated peptide, consisting of a sequence selected from the group consisting of SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 37, SEQ ID NO:
- 38, SEQ ID NO:
- 39, SEQ ID NO:
- 40, SEQ ID NO:
- 41, SEQ ID NO:
- 42, SEQ ID NO:
- 44, SEQ ID NO:
- 45, SEQ ID NO:
- 46, and SEQ ID NO:
- 47.
Dependencies are inferred from the claim text.