Color unmixing and region of interest detection in tissue samples
OwnerAndrew M. Rabinovichinventor
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Abstract
In a method and system and machine-readable medium, colors in an image (200) are unmixed (202) using a matrix X=AS, where A is a ns. ×nd matrix of spectral definitions, where ns is the number of spectral components and nd is the number of dyes into which the image is decomposed, and where S is a nd×l matrix of amounts of each dye at every pixel, where l is the number of pixels; the matrix X is constrained for solution by an unsupervised matrix decomposition method having constraints consistent with properties of an additive color model; and nd is determined. A texture is determined to identify areas of interest. Further processing may automatically measure cell dye concentration in the determined regions of interest.
Claims17
A method of spectral decomposition comprising computer-executed steps of:
- accessing an image comprising pixels, each pixel containing spectral data;
- creating a matrix X=AS, where A is a ns×nd matrix of spectral definitions, where ns is the number of spectral components and nd is the number of dyes into which the image is decomposed, and where S is a nd×l matrix of amounts of each dye at every pixel, where l is the number of pixels;
- constraining the matrix X for solution by an unsupervised matrix decomposition method having constraints consistent with properties of additive color model;
- and
- using the constrained matrix X to produce at least one image for at least one dye of the number of dyes.
An device for spectral decomposition comprising:
- a video processor to receive an input indicative of an image comprising pixels, each pixel containing spectral data;
- said processor adapted to create a matrix X=AS, where A is a ns×nd matrix of spectral definitions, where ns is the number of spectral components and nd is the number of dyes in to which the image is decomposed, and where S is a nd×l matrix of amounts of each dye at every pixel, where l is the number of pixels;
- to constrain the matrix X for solution by an unsupervised matrix decomposition method having constraints consistent with properties of an additive color model;
- and to produce at least one image for at least one dye of the number of dyes using the constrained matrix X.
Dependencies are inferred from the claim text.