Method for determining specific groups constituting heparins or low molecular weight heparins
AssigneeAventis Pharma S.A.
InventorsChristian Viskov, Pierre Mourier
Life of this patent
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Abstract
The invention provides methods for analyzing heparins, low-molecular-weight heparins, ultralow-molecular-weight heparins, and oligosaccharides by high performance liquid chromatography a stationary phase dynamically coated with a quaternary ammonium salt. The methods of the invention may be used to analyze samples without pre-treatment or to analyze samples that have been partially or exhaustively depolymerised and, optionally, reduced. Specific saccharides can be detected.
Claims25
A method of assaying a sample chosen from heparin, low-molecular-weight heparin, ultra low molecular weight heparin, and oligosaccharides, the method comprising
- analyzing the sample using a reversed phase strong anion exchange column coated with at least one cetyl trimethylammonium salt (CTA-SAX)
- wherein the analyzing comprises selectively detecting acetylated sugars by substracting an absorbance measured at a first wavelength at which both acetylated and non-acetylated sugars absorb from an absorbance measured at a second wavelength at which acetylated but not non-acetylated sugars absorb,
- wherein the first and second wavelengths are different, and
- wherein the two absorbances are measured using a UV detector to simultaneously detect absorbance at the first and the second wavelengths.
Dependencies are inferred from the claim text.