Method for producing cell culture substrate and apparatus for producing cell culture substrate

A method for producing a patterned cell culture substrate comprising:

• forming on a base material, a light shielding element containing a material which can shield ultraviolet rays or visible light, a photocatalyst-containing layer, and a cell adhesive layer containing a material to which cells adhere and which is decomposed or denatured by action of the photocatalyst upon irradiation with ultraviolet rays or visible light, wherein the photocatalyst is contained in between the cell adhesive layer and the light shielding element, and wherein the light shielding element is sandwiched between the base material and the photocatalyst-containing layer which contacts the cell adhesive material;
• irradiating the patterning substrate from the base material side to form a pattern consisting of a cell adhesion inhibiting portion in which the cell adhesive material is decomposed or denatured, and a cell adhesion portion which is other than the cell adhesion inhibiting portion;
• and
• attaching or adhering a cell to the cell adhesion portion, wherein the cell is in a cell culture.