Control system and method for flash separation
AssigneeBiotage AB
InventorsJeffrey A. Horsman, John R. Bickler
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Abstract
Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.
Claims20
A method of separating compounds in a sample using a column chromatography system, the method comprising:
- receiving data input sufficient for determining;
- at least two retention factors (Rf) corresponding to a primary compound and a secondary compound exposed to an initial solvent system;
- and
- a proportion of each solvent in the initial solvent system;
- generating a solvent ratio profile based on the received inputs including;
- a starting point corresponding to a starting solvent ratio;
- an ending point corresponding to an ending solvent ratio;
- and
- a transitional phase during which the solvent ratio increases from the starting solvent ratio to the ending solvent ratio;
- and
- introducing separation solvent system having the starting solvent ratio at a solvent flow rate into an upstream end of a chromatography column having a column volume;
- introducing a sample including the primary compound and the secondary compound into an upstream end of a chromatography column;
- varying the solvent ratio of the separation solvent system being introduced into the upstream end of the chromatography column in accord with the solvent ratio profile;
- and
- carrying at least the primary compound to a downstream end of the chromatography column.
Dependencies are inferred from the claim text.