Production of pharmaceutically active proteins in sprouted seedlings
AssigneeiBio, Inc. and Fraunhofer USA, Inc.
InventorsBurt D. Ensley, Vidadi Yusibov
Life of this patent
Estimating the term…
Abstract
The present invention provides systems and methods for producing pharmaceutically active proteins in transgenic sprouted seedlings. The transgenic sprouted seedlings of the invention are grown in a contained, regulatable environment, wherein expression of a pharmaceutically active protein is controlled by an exogenously inducible promoter. The transgenic sprouted seedlings may be eaten live or preferably harvested live to preserve the maximal biological activity of the pharmaceutically active protein.
Claims46
A method for producing pharmaceutically active proteins in sprouted seedling, comprising the steps of:
- growing a sprouted seedling in a contained, regulatable environment, wherein the sprouted seedling contains a viral transcript comprising (i) regulatory sequences that direct expression of the pharmaceutically active protein in the sprouted seedling;
- and (ii) a sequence that encodes a heterologous pharmaceutically active protein, wherein the pharmaceutically active protein is not produced as a fusion with a viral protein;
- and
- harvesting the sprouted seedling live, wherein the sprouted seedling has developed to the two-leaf stage prior to harvesting.
A method comprising steps of:
- growing a sprouted seedling in a contained, regulatable environment, wherein the sprouted seedling contains a viral expression cassette comprising (i) sequences of viral origin upstream and downstream of (ii) a gene encoding a pharmaceutically active protein, such that the pharmaceutically active protein is not produced as a fusion with a viral protein;
- and
- harvesting the sprouted seedling live, wherein the sprouted seedling has developed to the two-leaf stage prior to harvesting.
A method comprising steps of:
- growing a sprouted seedling in a contained, regulatable environment, wherein the sprouted seedling contains a viral expression cassette comprising (i) sequences of viral origin upstream and downstream of (ii) a gene encoding a pharmaceutically active protein, such that the pharmaceutically active protein is not produced as a fusion with a viral protein;
- and
- harvesting the sprouted seedling live, wherein the sprouted seedling has grown for 1-12 days post-germination prior to harvesting.
A method for producing pharmaceutically active proteins in a sprouted seedling, comprising the steps of:
- growing a sprouted seedling in a contained, regulatable environment, wherein the sprouted seedling contains a viral transcript comprising (i) regulatory sequences that direct expression of the pharmaceutically active protein in the sprouted seedling;
- and (ii) a sequence that encodes a heterologous pharmaceutically active protein, wherein the pharmaceutically active protein is not produced as a fusion with a viral protein;
- and
- harvesting the sprouted seedling live, wherein the sprouted seedling has grown for 1-12 days after germination prior to harvesting.
Dependencies are inferred from the claim text.