Synthesis and self-assembly of ABC triblock bola peptide amphiphiles

A method of making a self assembled micelle from triblock bola amphiphile with a lyophobic moiety capable of hydrogen bonding and having a first end and a second end;

• the first end of said lyophobic moiety chemically coupled to a first lyophilic head group;
• and the second end of said lyophobic moiety chemically coupled to a second lyophilic head group, wherein one lyophilic head group is selected from the group consisting of oligo(ethylene glycol) chains and cyclic oligo(ethylene glycols) chains, and the other lyophilic head group comprises an amino acid sequence comprising hydroxyl functionalities from L-serine, charged amino or carboxylic acid groups derived from aspartic acid or lysine, or the amino acid sequence selected from glutamic acid-glutamic acid-glutamic acid and lysine-lysine-lysine, comprising the steps of:
• making a first solution of the triblock bola-amphiphile in a charged ionic form;
• mixing the first solution with a second composition which changes the pH of the first solution towards a neutral pH;
• and reacting the first and second solutions until a gel forms.

A method of encapsulating a therapeutic agent comprising:

• providing a therapeutic agent;
• exposing said therapeutic agent to a bola amphiphile capable of self assembly, wherein the bola amphiphile comprises a lyophobic moiety capable of hydrogen bonding, the amphiphile having a first end and a second end;
• the first end of said lyophobic moiety chemically coupled to a first lyophilic head group;
• and the second end of said lyophobic moiety chemically coupled to a second lyophilic head group, wherein one lyophilic head group is selected from the group consisting of oligo(ethylene glycol) chains and cyclic oligo(ethylene glycols) chains, and the other lyophilic head group comprises an amino acid sequence comprising hydroxyl functionalities from L-serine, charged amino or carboxylic acid groups derived from aspartic acid or lysine, or the amino acid sequence selected from glutamic acid-glutamic acid-glutamic acid and lysine-lysine-lysine;
• and initiating self assembly.

A method of treating a patient in need thereof with a therapeutic agent encapsulated in a self assembled bola amphiphile, wherein the bola amphiphile comprises a lyophobic moiety capable of hydrogen bonding, the amphiphile having a first end and a second end;

• the first end of said lyophobic moiety chemically coupled to a first lyophilic head group;
• and the second end of said lyophobic moiety chemically coupled to a second lyophilic head group, wherein one lyophilic head group is selected from the group consisting of oligo(ethylene glycol) chains and cyclic oligo(ethylene glycols) chains, and the other lyophilic head group comprises an amino acid sequence comprising hydroxyl functionalities from L-serine, charged amino or carboxylic acid groups derived from aspartic acid or lysine, or the amino acid sequence selected from glutamic acid-glutamic acid-glutamic acid and lysine-lysine-lysine, comprising:
• identifying a site on a patient in need of a treatment;
• and administering the bola amphiphile encapsulated therapeutic agent to said site.

A method of encapsulating a nanotube comprising:

• forming a nanotube;
• exposing said nanotube to a bola amphiphile capable of self assembly and comprising a lyophobic moiety capable of hydrogen bonding, the bola amphiphile having a first end and a second end;
• the first end of said lyophobic moiety chemically coupled to a first lyophilic head group;
• and the second end of said lyophobic moiety chemically coupled to a second lyophilic head group, wherein one lyophilic head group is selected from the group consisting of oligo(ethylene glycol) chains and cyclic oligo(ethylene glycols) chains, and the other lyophilic head group comprises an amino acid sequence comprising hydroxyl functionalities from L-serine, charged amino or carboxylic acid groups derived from aspartic acid or lysine, or the amino acid sequence selected from glutamic acid-glutamic acid-glutamic acid and lysine-lysine-lysine, and initiating self assembly of said bola amphiphile.

A method of making a self assembled micelle from bola amphiphile with a hydrophobic moiety capable of hydrogen bonding, wherein the bola amphiphile has a first end and a second end;

• the first end of said lyophobic moiety chemically coupled to a first lyophilic head group;
• and the second end of said lyophobic moiety chemically coupled to a second lyophilic head group, wherein one lyophilic head group is selected from the group consisting of oligo(ethylene glycol) chains and cyclic oligo(ethylene glycols) chains, and the other lyophilic head group comprises an amino acid sequence comprising hydroxyl functionalities from L-serine, charged amino or carboxylic acid groups derived from aspartic acid or lysine, or the amino acid sequence selected from glutamic acid-glutamic acid-glutamic acid and lysine-lysine-lysine, comprising the steps of:
• making a first solution of the triblock bola-amphiphile in a charged ionic form;
• mixing the first solution with a second composition which changes the pH of the first solution towards a neutral pH;
• and reacting the first and second solutions until a gel forms.