Methods using novel chemiluminescent labels
AssigneeLumigen, Inc.
InventorsHashem Akhavan-Tafti, Renuka de Silva, Wenhua Xie
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Abstract
Methods using chemiluminescent label compounds and chemiluminescent labeled conjugates are provided. The compounds comprise an acridan ring bearing an exocyclic ketene dithioacetal group and further contain a labeling substituent which permits attachment to compounds of interest. The novel chemiluminescent compounds and labeled conjugates are convenient to prepare, are highly stable, and generate chemiluminescence rapidly on demand. The compounds and conjugates are useful in assays of an analyte in a sample and in assays employing labeled specific binding pairs.
Claims12
A method for detecting a labeled compound in a sample, wherein the labeled compound comprises a compound linked to a chemiluminescent label, said labeled compound having the formula:
- wherein each of R1, R2 and R3 is selected from substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted alkynyl, substituted or unsubstituted aryl, substituted or unsubstituted aralkyl groups of 1-20 carbon atoms, wherein R1 and R2 can be joined together in a ring, R4-R11 are independently selected from hydrogen and substituents which do not interfere with the generation of chemiluminescence, and at least one of R1-R11 is linked to the compound by means of L-RG, wherein L is a linking group and RG is a reactive group, said method comprising:
- a) reacting the labeled compound with a peroxide and a peroxidase for producing chemiluminescence from the chemiluminescent label wherein the ratio of labeled compound to peroxidase ranges from 100:1 to about 1:1000 and the peroxide is added in a concentration in the range of 10−6 M to 10−1 M to produce the chemiluminescence as a flash of light lasting not more than about five seconds;
- and
- b) detecting the chemiluminescence as an indication of the presence of said compound in said sample.
Dependencies are inferred from the claim text.