Holder for PCR sample collection and preparation
AssigneeSmiths Detection Inc.
InventorsDouglas Jason Green, Carrie Lynn Holmes
Life of this patent
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Abstract
A holder for PCR sample collection and preparation and method of using which has a buffer container housing removably connected to a plunger housing. A swab attached to an end of a plunger collects a sample of a specimen to be analyzed for biological warfare agents. The swab and plunger are inserted into the plunger housing, a buffer container is positioned inside the buffer container housing and the buffer container housing and plunger housing are attached. A buffer passes through the swab and elutes off the sample and the sample mixes with a reagent. The prepared sample loads into a reaction tube, by a whipping action, for analysis.
Claims8
A Polymerase Chain Reaction analysis preparation method comprising:
- providing a device including a swab and a plunger and wiping the swab over a test surface to collect a sample;
- inserting the swab into a buffer container by using a twisting action between a plunger housing and a buffer container housing to attach the plunger housing to the buffer container housing, thereby enabling a buffer to be released and pass through the swab and allowing the buffer and sample to pass into a chamber for mixing with a reagent mixture;
- and
- whipping the device, thereby loading a prepared sample into a reaction tube.
Dependencies are inferred from the claim text.