Apparatus for separating and purifying nucleic acid and method for separating and purifying nucleic acid

A device for separating and purifying nucleic acids comprising a combination of at least two or more apparatuses for separating and purifying nucleic acids, wherein each of said apparatuses comprises a pressure sensor, and the pressure in the accommodation part of each of said apparatuses can be independently detected,

• wherein each of said apparatuses is an apparatus for separating and purifying nucleic acids, which comprises:
• a cylindrical syringe having a leading end part in which a first opening part comprising an opening is formed, a base end part in which a second opening part comprising an opening is formed and an accommodation part between said first opening part and said second opening part, the accommodation part being able to hold a sample solution therein;
• a solid phase-holding member comprising a column with a circular shape, wherein the column comprises a top portion, a middle portion, and a bottom portion having a flow hole, and connects to said leading end part of said cylindrical syringe by the top portion of the column such that a part of the first opening part of the syringe is inside the column;
• a solid phase comprising an organic polymer having a hydroxyl group on the surface thereof, wherein the solid phase is accommodated in said solid phase-holding member, located only at the interior of the bottom portion of the column of the solid phase-holding member and contacts with the opening of the first opening part of the leading end part of the cylindrical syringe at the interior of the column, and adsorbs and desorbs nucleic acids in the sample solution;
• and
• a pressure difference-generating apparatus,
• wherein the pressure sensor capable of detecting the pressure in the accommodation part is connected to an operation part of the pressure difference-generating apparatus which couples to the opening of the second opening part.