Methods for detecting and analyzing N-glycolylneuraminic acid (Neu5Gc) in biological materials
AssigneeThe Regents of the University of California
InventorsAjit Varki, Pam Tangvoranuntakul, Nissi Varki, Elaine Muchmore, Pascal Gagneux, Sandra Diaz
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Abstract
The present application is in the field of sialic acid chemistry, metabolism and antigenicity. More particularly, the present invention relates to the detection and analysis of the non-human sialic acid, N-glycolylneuraminic acid (Neu5Gc) in bio-logical materials, such as food and clinical specimens. Such detection and analysis is facilitated by the use of Neu5Gc specific antibodies. The present invention also relates to the detection of anti Neu5Gc antibodies in clinical samples, as well as the production of anti-Neu5Gc specific antibodies.
Claims14
A method of detecting an amount of anti-Neu5Gc specific antibodies in a fluid sample comprising:
- (a) determining the amount of anti-Neu5Ac antibodies present in the fluid sample using Neu5Ac as an antigen target;
- (b) determining the amount of anti-Neu5Gc antibodies present in the fluid sample using Neu5Gc as an antigen target;
- and
- (c) subtracting the amount of anti-Neu5Ac antibodies from the amount of anti-Neu5Gc antibodies to determine the amount of anti-Neu5Gc specific antibodies.
Dependencies are inferred from the claim text.