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US 7,682,511 B2Granted
Method and apparatus for the filtration of biological solutions
AssigneeSpf Innovations, LLC
InventorsGaston de los Reyes, Leon Mir
Life of this patent
Estimating the term…
Abstract
A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.
Claims26
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a separation module and having at least one channel having specific membrane area greater than about 40 cm-1;
- operating the separation module in a single pass tangential flow filtration mode;
- wherein the separation module comprises:
- a stacked configuration comprising:
- a first plurality of cassettes, each cassette having a feed manifold, a retentate manifold, at least one permeate channel, and at least one flow channel fluidly coupled to the feed manifold and to the retentate manifold, the flow channels of the plurality of cassettes fluidly coupled in parallel;
- a staging plate disposed adjacent the first plurality of cassettes;
- at least one second cassette having a feed manifold and a retentate manifold, the staging plate disposed between the first plurality of cassettes and the at least one second cassette;
- and
- wherein the staging plate fluidly couples the retentate manifold of one of the cassettes of the first plurality of cassettes to the feed manifold of the second cassette and blocks the flow from the feed manifold of one of the cassettes of the first plurality of cassettes, thereby serializing the retentate flow through the system.
6 more elements
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1;
- operating the separation module in a single pass tangential flow filtration mode;
- and
- setting a conversion factor to a predetermined factor by controlling a ratio of the feed stream flow rate to the retentate stream flow rate.
1 more element
A method for filtering a liquid feed stream comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1 and operating the separation module in a single pass tangential flow filtration mode,
- wherein operating the separation module in a single pass tangential flow filtration mode further comprises controlling at least two of:
- a feed flow rate;
- a retentate flow rate;
- a permeate flow rate;
- the specific feed flow rate;
- a transmembrane pressure (TMP) of the at least one channel;
- and
- a transchannel pressure (TCP) of the at least one channel;
- and
- further comprising selecting a conversion factor and iteratively adjusting at least one of:
- the feed pressure into the at least one channel;
- and
- a retentate pressure of the at least one channel;
- in response to measuring the conversion.
12 more elements
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1;
- operating the separation module in a single pass tangential flow filtration mode;
- and
- wherein the module further comprises a plurality of stages having a plurality of channels having specific membrane areas greater than about 40 cm−1 wherein the module is an internally staged filtration separation module, such that the retentate from each stage except the last stage becomes the feed stream of each succeeding stage.
1 more element
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1;
- operating the separation module in a single pass tangential flow filtration mode;
- and
- wherein the module further comprises a plurality of stages having a plurality of channels and at least one diafiltrate distributor having a plurality of diafiltration flow passages, and further comprising adding a diafiltrate through the at least one diafiltrate distributor and distributing the diafiltrate to corresponding ones of the plurality of channels.
1 more element
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1;
- operating the separation module in a single pass tangential flow filtration mode;
- and
- wherein the module is an internally staged filtration separation module, such that the retentate from each stage except the last stage becomes the feed stream of each succeeding stage.
1 more element
A method for filtering a liquid feed comprising:
- continuously supplying the feed stream at a specific feed flow rate of less than about 200 lmh into a membrane separation module and having at least one channel having specific membrane area greater than about 40 cm−1;
- operating the separation module in a single pass tangential flow filtration mode;
- and further comprising
- pumplessly supplying the feed stream from a pressurized feed tank;
- coupling the feed tank to a pressure source;
- and
- controlling the pressure of the feed tank.
4 more elements
Dependencies are inferred from the claim text.