Method of sterilization
AssigneeGE Healthcare Bio-Sciences AB
InventorsJan Berglof, Ingrid Drevin, Ola Lind, Ulf Segerbo
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Abstract
The present invention relates to a method of sterilizing a chromatography column which is packed with a separation matrix, which method comprises passing a superheated aqueous liquid through the packing of the column to obtain at least one substantially sterile packed chromatography column. The method may also comprise a preceding heating. In the most advantageous embodiment, the superheated aqueous liquid is passed in a substantially uniform flow through the packing of the column, resulting in a sterility of the packed column.
Claims14
A method of sterilizing at least one chromatography column packed with a separation matrix, comprising:
- providing a sealed chromatography column made from a material which withstands the temperature and pressure conditions used;
- applying a pressure over the chromatography column;
- heating an aqueous liquid which is a buffer commonly used in chromatography to a temperature which under the prevailing pressure provides a superheated aqueous liquid;
- and
- passing said superheated aqueous liquid through the packing of said column(s) to obtain at least one substantially sterile packed chromatography column, wherein the temperature and pressure of the superheated aqueous liquid are substantially maintained throughout the column such that said aqueous liquid is maintained superheated.
Dependencies are inferred from the claim text.