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US 7,678,960 B2Granted
Generation of plants with altered oil, protein, or fiber content
AssigneeAgrinomics LLC
InventorsJohn P. Davies, Hein Tsoeng (Medard) Ng
Life of this patent
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Abstract
The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an HIO nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.
Claims13
A method of producing oil comprising:
- growing a transgenic plant that comprises a heterologous polynucleotide that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
- 12 and conveying an increased oil phenotype on said transgenic plant when expressed in said plant, whereby the transgenic plant has said increased oil content phenotype, relative to a plant of the same species that does not comprise said heterologous polynucleotide;
- and
- recovering oil from said transgenic plant.
1 more element
A method of producing increased oil content in a plant, said method comprising:
- introducing into progenitor cells of the plant a heterologous constitutive promoter operatively linked to a heterologous polynucleotide that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:
- 12 and conveying an increased oil phenotype on said plant cells when expressed in said plant cells, to produce transformed cells overexpressing the polypeptide;
- growing the transformed progenitor cells to produce a transgenic plant, wherein said heterologous polynucleotide is expressed in said transgenic plant;
- identifying said transgenic plant that exhibits the increased oil content phenotype relative to a plant of the same species that does not comprise said heterologous polynucleotide;
- and
- recovering oil from said transgenic plant.
3 more elements
A method of producing oil comprising:
- growing a transgenic plant that comprises a heterologous polynucleotide that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
- 12 and conveying a decreased fiber content phenotype and an increased oil phenotype on said plant when expressed in said plant;
- identifying the transgenic plant that has said decreased fiber content phenotype and said increased oil phenotype, relative to a plant of the same species that does not comprise the heterologous polynucleotide;
- and
- recovering oil from said transgenic plant.
2 more elements
Dependencies are inferred from the claim text.