siRNA targeting serine/threonine kinase 12 (STK12 or aurora B kinase)
AssigneeDharmacon, Inc.
InventorsAnastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Steven Read, Stephen Scaringe
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Abstract
Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to STK12.
Claims15
An siRNA molecule consisting of:
- (a) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region consisting of 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ ID NO:
- 490;
- and (b) either no overhang regions or at least one overhang region, wherein each overhang region is six or fewer nucleotides in length.
A chemically synthesized double stranded siRNA molecule that directs cleavage of an mRINA via RNA interference, wherein:
- (a) each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length;
- and
- (b) one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ ID NO:
- 490.
An siRNA molecule, wherein said siRNA molecule consists of:
- p1 (a) a sense region, wherein said sense region consists of a sequence that is SEQ ID NO:
- 490, and an antisense region, wherein said antisense region consists of a sequence that is the complement of SEQ ID NO:
- 490;
- and (b) either no overhang regions or at least one overhang region, wherein each overhang region is six or fewer nucleotides in length.
Dependencies are inferred from the claim text.