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US 7,678,892 B2Granted
Dye-labeled ribonucleotide triphosphates
AssigneeApplied Biosystems, LLC
InventorsPeter Virgil Fisher, Paolo Vatta, Shaheer H. Khan
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Abstract
The invention provides novel dye-labeled ribonucleotide analogs and methods for synthesizing those analogs. The compounds of the invention are especially useful for DNA sequencing by the polymerase chain reaction.
Claims13
A compound having the formula:
- wherein B is a nucleobase;
- L is a linker selected from a propargyl-ethyl-oxide-amino linker, a propargyl linker, a benzylamine linker, a rigid linker, a tuned linker, and a heterocycle linker;
- R3 is triphosphate, α-thiotriphosphate, or a salt thereof, and Dye is a fluorescein-type dye, a rhodamine-type dye, an energy transfer dye pair, or a cyanine-type dye.
A compound of the formula II:
- wherein L is a linker selected from a propargyl-ethyl-oxide-amino linker, a propargyl linker, a propargyl-propyl-oxide-amino linker, a benzylamine linker, a rigid linker, and a multimer thereof;
- wherein R4 is either NH2, OH, or O, and R5 is either NH2, OH, or H;
- wherein R3 is either triphosphate, α-thiotriphosphate, or a salt thereof;
- and
- wherein the dye is any reporter group.
2 more elements
A compound having the formula:
- wherein B is a nucleobase selected from uracil, cytosine, adenine, 7-deazaadenine, guanine, and 7-deazaguanine;
- wherein R3 is triphosphate or a salt thereof;
- wherein L is a linker selected from propargyl-ethyl-oxide-amino and propargyl wherein the linker is attached to the 8-C of a adenine, 7-deazaadenine, guanine, or 7-deazaguanine nucleobase, the 7-C or 8-C of a 7-deazaadenine or 7-deazaguanine nucleobase, or the C-5 of a uracil or cytosine nucleobase;
- and
- wherein Dye is selected from a rhodamine-type dye and a fluorescein-type dye.
2 more elements
Dependencies are inferred from the claim text.