Method of lysis of erythrocytes
AssigneeImmunotech
InventorsAndre van Agthoven, Jean-Pierre Daziano, John Allen Maples
Life of this patent
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Abstract
A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of CO2 by the erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.
Claims21
A method of analyzing leukocytes using flow cytometry comprising:
- (a) mixing a whole blood sample with a lysis reagent comprising a carbonic anhydrase, and an ammonium salt or a heterocyclic amine or salt thereof, forming a mixture;
- (b) incubating said mixture for a predetermined period of time sufficient to lyse erythrocytes while preserving leukocytes in said mixture and maintaining morphology and viability of said leukocytes;
- and
- (c) analyzing said leukocytes in said mixture on a flow cytometer.
A method of analyzing leukocytes using flow cytometry comprising:
- (a) mixing a whole blood sample with a lysis reagent comprising an ammonium salt or a heterocyclic amine or salt thereof and a catalyst agent enabling inducing formation of carbamate in the presence of erythrocytes, forming a mixture;
- (b) incubating said mixture for a predetermined period of time, wherein said catalyst agent induces formation of carbamate in said mixture, thereby causing sufficient lysis of erythrocytes while preserving leukocytes in said mixture and maintaining morphology and viability of said leukocytes;
- and
- (c) analyzing said leukocytes in said mixture on a flow cytometer.
Dependencies are inferred from the claim text.