Procedure for the large-scale T-lymphocytes culture in a homogeneous system

A process for amplifying TALL-104 lymphocytes in a homogeneous culture system within a multi-chamber stack comprising:

• adding into the multi-chamber stack an inoculum of at least 0.7×106 TALL-104 cells/ml in an initial volume from 1/10 to ⅙ of the multi-chamber stack volume capacity and the same volume of fresh antibiotic-free complete medium, wherein said medium comprises interleukin-2 (IL-2);
• amplifying the cell number by adding a volume of said complete medium corresponding to the volume contained in the multi-chamber stack every 3-5 days;
• and
• harvesting at least 1×109 TALL-104 cells grown in homogeneous conditions.