Addressable nanopores and micropores including methods for making and using same

A process to fabricate nanopores and micropores, comprising the steps of:

• providing an integral substrate member having a thickness and first and second opposing surfaces;
• forming at least one first V-shaped channel lengthwise in a first direction in said first surface;
• and
• forming at least one second V-shaped channel lengthwise in a second direction in said second surface, said second direction being disposed at an angle relative to said first direction,
• wherein said first channel and said second channel extend inwardly from said first and second surfaces and intersect at a point, said point defining a pore extending through said substrate member from said first surface to said second surface;
• placing a first crystal into said first channel adjacent said pore;
• placing a second crystal into said second channel adjacent said pore;
• applying a force to urge said first and second crystals towards one another to maintain their position relative to one another;
• placing an uncured liquid polymer material into said first and second channels adjacent said first and second crystals;
• curing said polymer material to provide a plurality of polymer molecules, the polymer molecules each having a diameter;
• and
• removing said crystals from said pore, said pore now having a size defined by the point of intersection between said first and second crystals and the diameter of one of said plurality of polymer molecules.