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AssigneeThe Florida State University Research Foundation, Inc.
InventorsTimothy Cross, William W. Brey, Alexej Smirnov, Eduard Y Chekmenev
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Disclosed is a method for detection of ligand-cell membrane protein binding by solid state NMR spectroscopy. The method starts by forming a lipid bilayer inside nanopores of an anodic aluminum oxide (AAO) substrate, the lipid bilayer containing a membrane protein sample. The AAO substrate is treated with multiple candidate ligands having potential binding affinity for the membrane protein. Solid-state NMR analysis is performed on the treated AAO/lipid preparation so as to generate an NMR spectrum for the treated membrane protein. The solid-state NMR spectrum of the treated membrane protein is compared with the spectrum of the same preparation of membrane protein in the absence of the ligands. It is then determined whether the solid-state NMR spectrum of the treated membrane protein has shifted from the NMR spectrum of the untreated membrane protein, a shift being indicative of protein binding by the candidate ligand.
A method for detecting a candidate drug binding to a cell membrane protein by solid-state NMR, the method comprising:
A method for detecting a candidate drug binding to a cell membrane protein by shifting solid-state NMR spectra of the protein, the method comprising:
Dependencies are inferred from the claim text.