Method for amplifying genomic DNA
AssigneeNational Institute of Radiological Sciences
InventorsTakashi Imai, Mayumi Iwakawa, Yuichi Michikawa
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Abstract
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies/5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
Claims11
A method for amplifying genomic DNA comprising the steps of:
- (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45° to 80° C. and producing a genomic DNA-dispersed agarose solution thereby forming a dispersion of 0.002 to 1 copies/5 microliter of spatially separated single-stranded genomic DNA, then
- (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing the pH of the gel;
- and then
- (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
Dependencies are inferred from the claim text.