Arrays of biological membranes and methods and use thereof
AssigneeCorning Incorporated
InventorsYe Fang, Anthony G. Frutos, Steven J. Jonas, Peter J. Kalal, Joydeep Lahiri
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Abstract
The present invention overcomes the problems and disadvantages associated with prior art arrays by providing an array comprising a plurality of biological membrane microspots associated with a surface of a substrate that can be produced, used and stored, not in an aqueous environment, but in an environment exposed to air under ambient or controlled humidities. Preferably, the biological membrane microspots comprise a membrane bound protein. Most preferably, the membrane bound protein is a G-protein coupled receptor, an ion channel, a receptor serine/threonine kinase or a receptor tyrosine kinase.
Claims18
A method for detecting a binding event between a probe and target compound, said method comprising:
- contacting a solution comprising the target compound with an air-dried array of immobilized probe biological membrane microspots, at least one of the biological membrane microspots comprising at least one G-protein coupled receptor, wherein the membrane microspots are directly and noncovalently associated with a γ-aminopropyl-silane layer disposed directly on a surface of a glass substrate, such that the microspots remain adsorbed on said layer and retain biological function when drawn through an air-water interface, the target compound having one or more constituents;
- and
- detecting a binding event between at least one or more of the probes and one or more of the constituents of the target.
Dependencies are inferred from the claim text.