Method of coating fine particles with lipid film
AssigneeKyowa Hakko Kirin Co., Ltd.
InventorsYasuki Kato, Masahiro Yamauchi, Hiroko Kusano, Takeshi Iwata, Takaaki Uochi, Shiro Akinaga
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Abstract
An object of the present invention is to provide a safe, convenient and efficient method for coating fine particles with lipid membrane. There is provided a method for coating fine particles with lipid membrane, characterized in that, the rate of a polar organic solvent in an aqueous solution containing the polar organic solvent where the fine particles are dispersed and lipid is dissolved, is decreased.
Claims13
A method for coating a complex of a nucleic acid and a liposome with closed vesicles of a lipid bilayer, the liposome comprising cationic lipid, the complex obtained by mixing the nucleic acid and the liposome in water, which consists of the steps of (i) and (ii):
- (i) suspending the complex in an aqueous solution consisting essentially of:
- water,
- polar organic solvent(s) selected from an alcohol, a glycol and a polyalkylene glycol, and
- lipid(s) selected from phospholipid, glyceroglycolipid, sphingoglycolipid, cholesterol and synthetic lipid, excluding a cationic lipid,
- wherein the complex has a diameter of 10 nm to 1,000 nm, and
- wherein the lipid(s) is dissolved in the aqueous solution;
- and
- (ii) adding water to the aqueous solution in which the complex is suspended, so as to decrease the vol % concentration of the polar organic solvent(s) in the aqueous solution, whereby the complex is coated with closed vesicles of the lipid bilayer formed of the lipid(s).
A method for coating a complex of a nucleic acid and a liposome with closed vesicles of a lipid bilayer, the liposome comprising cationic lipid, the complex obtained by mixing the nucleic acid and the liposome in water, which consists of the steps of (i), (ii), (iii) and (iv):
- (i) suspending the complex in liquid A consisting essentially of:
- water, and
- polar organic solvent(s) selected from an alcohol, a glycol and a polyalkylene glycol,
- wherein the complex has a diameter of 10 nm to 1,000 nm,
- (ii) dissolving lipid(s) selected from phospholipid, glyceroglycolipid, sphingoglycolipid, cholesterol and synthetic lipid, excluding a cationic lipid, in liquid B consisting essentially of:
- polar organic solvent(s) selected from an alcohol, a glycol and a polyalkylene glycol, and optionally water,
- (iii) mixing the liquid A and the liquid B, to obtain liquid C in which the complex is suspended, and
- (iv) adding water to the liquid C so as to decrease the vol % concentration of the polar organic solvent(s), to obtain liquid D, and whereby the complex is coated with closed vesicles of the lipid bilayer formed of the lipid(s).
Dependencies are inferred from the claim text.