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US 7,678,394 B2Granted
Analytical methods for identifying ginseng varieties
OwnerBaihua Shen, Zhongkai Yan +1inventors
InventorsBaihua Shen, Zhongkai Yan, Heming Niu
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Abstract
An analytical method for identifying woods-grown ginseng and mountain cultivated ginseng comprise: (a) preparing ginseng extracts, (b) acquiring fingerprint regions by GC-MS analysis, (c) selecting at least 8 GC peaks for similarity calculation; (d) calculating similarity coefficients; and (e) comparing similarity coefficients of samples to those of standards.
Claims12
A method for distinguishing between woods-grown ginseng and mountain-cultivated ginseng, comprising:
- (a) preparing a ginseng extract of a woods-grown ginseng or mountain-cultivated ginseng sample;
- (b) obtaining a GC-MS spectrum of said extract;
- (c) calculating a similarity coefficient based on at least 8 peaks in the GC-MS spectrum with respect to a selected standard;
- said selected standard being a set of at least 8 peaks in GC-MS spectrum of known woods-grown ginseng;
- and
- (d) comparing the similarity coefficient calculated in step (c) to pre-determined standard similarity coefficients of (i) known mountain-cultivated ginseng with respect to known woods-grown ginseng and (ii) known woods-grown ginseng with respect to known woods-grown ginseng, based on at least 8 peaks in GC-MS spectrum.
3 more elements
A method for distinguishing between woods-grown ginseng and mountain-cultivated ginseng, comprising:
- (a) preparing a ginseng extract of a woods-grown ginseng or mountain-cultivated ginseng sample;
- (b) obtaining a GC-MS spectrum of said extract;
- (c) calculating a similarity coefficient based on at least a first set of 8 peaks in the GC-MS spectrum with respect to a selected standard;
- said selected standard being a second set of at least 8 peaks in GC-MS spectrum of known woods-grown ginseng, said second set of at least 8 peaks being selected from peaks corresponding to:
- 5-methyltetradecane, 5-isobutylnonane, 5-butylnonane, β-panasinsene, p-acetylacetophenone, paeonal, [1aR-(1aα,7α,7aα,7bα)]-1a,2,3,5,6,7,7a,7b-Octahydro-(1,1,7,7a-tetramethyl-1H-cyclopropa[a]naphthalene, α-caryophyllene, [1aR-(1aα.,4aα,7α,7aβ,7bα)]-Decahydro-1,1,7-trimethyl-4-methylene-1H-cycloprop[e]azulene, isocaryphyilene, 8-methylheptadecane, n-elcosane, crocetane, falcarinol, cis,cis-linoleic acid;
- and
- (d) comparing the similarity coefficient calculated in step (c) to pre-determined standard similarity coefficients of (i) known mountain-cultivated ginseng with respect to known woods-grown ginseng and (ii) known woods-grown ginseng with respect to known woods-grown ginseng, based on at least 8 peaks in GC-MS spectrum.
4 more elements
A method for distinguishing between woods-grown ginseng and mountain-cultivated ginseng, comprising:
- (a) preparing a ginseng extract of a woods-grown ginseng or mountain-cultivated ginseng sample, said sample being of a plant part selected from a main root, a lateral root, or a rootlet;
- (b) obtaining a GC-MS spectrum of said extract;
- (c) calculating a similarity coefficient based on at least a first set of 8 peaks in the GC-MS spectrum with respect to a selected standard;
- said selected standard being a second set of at least 8 peaks in GC-MS spectrum of known woods-grown ginseng of the same plant part as in step (a), said second set of at least 8 peaks being selected from peaks coffesponding to:
- 5-methyltetradecane, 5-isobutylnonane, 5-butylnonane, β-panasinsene, p-acetylacetophenone, paeonal, [1aR-(1aα,7α,7aα,7bα)]-1a,2,3,5,6,7,7a,7b-Octahydro-1,1,7,7a-tetramethyl-1H-cyclopropa[a]naphthalene, α-caryophyllene, [1aR-(1aα.,4aα,7α,7aβ,7bα)]-Decahydro-1,1,7-trimethyl-4-methylene-1H-cycloprop[e]azulene, isocaryphyilene, 8-methylheptadecane, n-elcosane, crocetane, falcarinol, cis,cis-linoleic acid;
- and
- (d) comparing the similarity coefficient calculated in step (c) to pre-determined standard similarity coefficients of (i) known mountain-cultivated ginseng with respect to known woods-grown ginseng, each of the same plant part as in step (a), and (ii) known woods-grown ginseng with respect to known woods-grown ginseng, each of the same plant as in step (a), based on at least 8 peaks in GC-MS spectrum.
4 more elements
Dependencies are inferred from the claim text.