Viral vectors expressing fusion of viral large envelope protein and protein of interest
AssigneeHepgenics Pty Ltd
InventorsDavid Andrew Anderson, Elizabeth Vera Ludmila Grgacic
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Abstract
The present invention provides a virus-like particle (VLP) comprising i) a polypeptide comprising a polypeptide of interest (POI) and at least a particle-associating portion of a large envelope (L) polypeptide of an avian hepadnavirus or a functional derivative or homolog thereof, and ii) a small envelope (S) polypeptide of an avian hepadnavirus or a functional derivative or homolog thereof. By introducing one or more POIs into the L polypeptide, the POI is translocated along with L into a particle structure made up primarily of S polypeptide. The present invention furthermore provides methods for producing a recombinant virus-like particle.
Claims6
A fusion polypeptide comprising a polypeptide of interest (POI) fused to a particle-associating portion of a large envelope polypeptide (L) of an avian hepadnavirus wherein the POI is not a pre-S region of an avian hepadnavirus and is located N-terminally to said particle-associating portion of the L polypeptide, and wherein the particle-associating portion consists of a fragment of the L polypeptide, wherein said fragment is (i) the S domain, (ii) the S domain minus the TM1 domain, or (iii) a portion of the L polypeptide which is downstream of TM1 and comprises at least TM2, the 5′ cysteine loop between TM1 and TM2, and the sequence downstream of TM2.
Dependencies are inferred from the claim text.