Reagent compositions for use in electrochemical detection
AssigneeHome Diagnostics, Inc.
InventorsDouglas E. Bell, Michele Albino
Life of this patent
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Abstract
Biological reagent compositions with improved sensitivity to the concentration of blood glucose in patient samples for use in measuring systems and methods. The reagent compositions comprise a glucose oxidoreductase enzyme, a flavin nucleoside coenzyme and a mediator formulation. The mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex.
Claims64
A reagent for detecting an analyte, comprising:
- a glucose oxidoreductase;
- flavin adenine dinucleotide;
- and
- a mediator formulation comprising at least one electroactive organic molecule independently selected from a phenoxazine or a phenoxazinium salt, and at least one coordination complex.
A reagent for detecting an analyte, comprising:
- a glucose oxidoreductase;
- flavin adenine dinucleotide;
- and
- a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one coordination complex independently selected from iron, osmium, or ruthenium complexes.
A reagent for detecting an analyte, comprising:
- a glucose oxidoreductase;
- a coenzyme selected from a flavin nucleoside and a nicotinamide nucleotide;
- and
- a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and ruthenium hexamine.
A reagent for detecting an analyte, comprising:
- a glucose oxidoreductase;
- a coenzyme selected from a flavin nucleoside;
- and
- a mediator formulation comprising at least one electroactive organic molecule independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine;
- and ruthenium hexamine.
A reagent for detecting an analyte, comprising:
- glucose dehydrogenase;
- a coenzyme selected from flavin adenine dinucleotide;
- and
- a mediator formulation comprising at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at ruthenium hexamine.
An electrochemical sensor strip, comprising:
- a base;
- a working electrode;
- a counter electrode;
- and
- at least one reagent layer, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic molecule independently selected from a phenoxazine or a phenoxazinium salt and at least one coordination complex.
An electrochemical sensor strip, comprising:
- a base;
- a working electrode;
- a counter electrode;
- and
- at least one reagent layer, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one coordination complex independently selected from iron, osmium and ruthenium complexes.
An electrochemical sensor strip, comprising:
- a base;
- a working electrode;
- a counter electrode;
- and
- at least one reagent layer, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic compound independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one ruthenium coordination complex.
An electrochemical sensor strip, comprising:
- a base;
- a working electrode;
- a counter electrode;
- and
- at least one reagent layer, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex, wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine, and the at least one coordination complex is ruthenium hexamine.
An electrochemical sensor strip, comprising:
- a base;
- a working electrode;
- a counter electrode;
- and
- at least one reagent layer, wherein the at least one reagent layer comprises glucose dehydrogenase, a coenzyme selected from flavin adenine dinucleotide, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one ruthenium coordination complex.
A method of making an electrochemical sensor strip, comprising:
- depositing a first electrode on a base;
- depositing a second electrode on the base;
- and
- applying a reagent layer on the base covering at least a portion of at least first or second electrode, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic molecule independently selected from a phenoxazine or a phenoxazinium salt, and at least one coordination complex.
A method of making an electrochemical sensor strip, comprising:
- depositing a first electrode on a base;
- depositing a second electrode on the base;
- and
- applying a reagent layer on the base covering at least a portion of at least first or second electrode, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one at least one electroactive organic molecule independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one coordination complex independently selected from iron, osmium or ruthenium complexes.
A method of making electrochemical sensor strip, comprising:
- depositing a first electrode on a base;
- depositing a second electrode on the base;
- and
- applying a reagent layer on the base covering at least a portion of at least first or second electrode, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic compound independently selected from a benzophenoxazine or a benzophenoxazinium salt, and at least one ruthenium coordination complex.
A method of making an electrochemical sensor strip, comprising:
- depositing a first electrode on a base;
- depositing a second electrode on the base;
- and
- applying a reagent layer on the base covering at least a portion of at least first or second electrode, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, wherein the mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex, wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine, and the at least one coordination complex is ruthenium hexamine.
A method of making an electrochemical sensor strip, comprising:
- depositing a first electrode on a base;
- depositing a second electrode on the base;
- and
- applying a reagent layer on the base covering at least a portion of at least first or second electrode, wherein the at least one reagent layer comprises glucose dehydrogenase, a coenzyme selected from flavin adenine dinucleotide, and a mediator formulation, wherein the mediator formulation comprises at least one benzophenoxazine or benzophenoxazinium salt and at least one ruthenium coordination complex.
A meter for use in combination with a test strip for measuring a glucose level in a blood sample applied to a sample chamber in a test strip, said test strip comprising a base, a working electrode, a counter electrode, and at least one reagent layer, wherein the at least one reagent layer comprises glucose dehydrogenase, a coenzyme selected from flavin adenine dinucleotide, and a mediator formulation, said mediator formulation comprising at least one electroactive organic molecule and at least one coordination complex, wherein the at least one electroactive organic molecule is independently selected from a benzophenoxazine or a benzophenoxazinium salt, and the at least one at least one coordination complex is a ruthenium complex, said meter comprising:
- a first connector for receiving said test strip;
- a second connector for receiving a removable data storage device;
- a processor;
- a display;
- and
- at least one electrode for applying at least a first voltage between said working and counter electrodes when said test strip is inserted.
A meter for use in combination with a test strip for measuring a glucose level in a blood sample applied to a sample chamber in a test strip, said test strip comprising a base, a working electrode, a counter electrode, and at least one reagent layer, wherein the at least one reagent layer comprises a glucose oxidoreductase, a coenzyme selected from a flavin nucleoside, and a mediator formulation, said mediator formulation comprising at least one electroactive organic molecule and at least one coordination complex, wherein the at least one electroactive organic molecule is independently selected from 9-(dimethylamino)benzophenoxazin-7-ium chloride, N-(9H-benzophenoxazin-9-ylidene)-N-methylmethanaminium chloride, 8-dimelthylamino-2,3-benzophenoxazine hemi (zinc chloride) salt, 7-dimethylamino-1,2-benzophenoxazine or 8-dimethylamino-2,3-benzophenoxazine, and the at least one coordination complex is ruthenium hexamine, said meter comprising:
- a first connector for receiving said test strip;
- a second connector for receiving a removable data storage device;
- a processor;
- a display;
- and
- at least one electrode for applying at least a first voltage between said working and counter electrodes when said test strip is inserted.
Dependencies are inferred from the claim text.