Infectious, chimeric hepatitis C virus, methods of producing the same and methods of use thereof
AssigneeThe Rockefeller University
InventorsCharles Rice, Brett D Lindenbach, Matthew J Evans, Christopher Jones
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Abstract
The present invention provides infectious recombinant Hepatitis C Viruses (HCV), and vectors, cells and animals comprising the same. The present invention provides methods of producing infectious recombinant HCV, and their use in identifying anti-HCV therapeutic agents, as well as sequences of HCV associated with HCV pathogenesis.
Claims31
An isolated nucleic acid comprising a chimeric Hepatitis C Virus (HCV) genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from HCV strain H77 a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from HCV strain JFH-1, wherein said chimeric HCV genome comprises at least one mutation selected for enhanced capacity to produce infectious virus, and wherein said chimeric HCV genome is infectious.
A method for producing infectious HCV, comprising contacting a cell with an isolated nucleic acid comprising a chimeric HCV genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from HCV strain H77, a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from HCV strain JFH-1, wherein said chimeric HCV genome comprises at least one mutation selected for enhanced capacity to produce infectious virus, and wherein said chimeric HCV genome is infectious.
A viral particle comprising a chimeric Hepatitis C Virus (HCV) genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from a first HCV strain, a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from a second HCV strain, wherein said first HCV is a J6 strain, wherein said second HCV strain is strain JFH-1, and wherein said viral particle is infectious HCV.
An isolated nucleic acid comprising a chimeric Hepatitis C Virus (HCV) genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from HCV strain J6, a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from HCV strain JFH-1, and wherein said chimeric HCV genome is infectious.
A method for producing infectious HCV, comprising contacting a cell with an isolated nucleic acid comprising a chimeric HCV genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from HCV strain J6, a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from HCV strain JFH-1, and wherein said chimeric HCV genome is infectious.
A viral particle comprising a chimeric Hepatitis C Virus (HCV) genome, wherein said chimeric HCV genome comprises the structural core, E1 and E2 genes and nonstructural p7 and NS2 genes from a first HCV strain, a 5′ non-coding region (NCR), nonstructural NS3, NS4A, NS4B, NS5A, NS5B genes and a 3′ non-coding region (NCR) from a second HCV strain, wherein said first HCV is an H77 HCV strain, wherein said second HCV strain is strain JFH-1, wherein said chimeric HCV genome comprises at least one mutation selected for enhanced capacity to produce infectious virus, and wherein said viral particle is infectious HCV.
Dependencies are inferred from the claim text.