Chimeric immunogens
AssigneeSanofi Pasteur Limited
InventorsMichel H. Klein, Run-Pan Du, Mary E. Ewasyshyn
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Abstract
Multimeric hybrid genes encoding the corresponding chimeric protein comprise a gene sequence coding for an antigenic region of a protein from a first pathogen linked to a gene sequence coding for an antigenic region of a protein from a second pathogen. The pathogens particularly are parainfluenza virus (PIV) and respiratory syncytial virus (RSV). A single recombinant immunogen is capable of protecting infants and similar susceptible individuals against diseases caused by both PIV and RSV.
Claims16
A multimeric hybrid gene encoding a chimeric protein including a protein from parainfluenza virus (PIV) and a protein from respiratory syncytial virus (RSV), comprising a nucleotide sequence encoding a PIV-3 F protein or a fragment thereof having fusion activity linked to a nucleotide sequence coding for a RSV F protein or a fragment thereof having fusion activity.
A process for the preparation of a chimeric protein including a protein from parainfluenza virus (PIV) and a protein from respiratory syncytial virus (RSV), which comprises:
- isolating a first nucleotide sequence encoding a PIV-3 F protein or a fragment thereof having hemagglutinin-neurominidase activities,
- isolating a second nucleotide sequence encoding a RSV F protein or a fragment thereof having fusion activity,
- linking said first and second nucleotide sequences to form a multimeric hybrid gene, and
- expressing the multimeric hybrid gene in a cellular expression system.
Dependencies are inferred from the claim text.