Optimized Fc variants and methods for their generation

A protein comprising an Fc variant of a parent Fc polypeptide, wherein said Fc variant comprises an amino acid modification selected from the group consisting of 332A, 332D, 332E, 332F, 332G, 332H, 332K, 332L, 332M, 332N, 332P, 332Q, 332R, 332S, 332T, 332V, 332W and 332Y, wherein numbering is according to the EU index, and wherein said Fc variant comprises an engineered glycoform.

A protein comprising an Fc variant of a parent Fc polypeptide, wherein said Fc variant comprises an amino acid modification at position 332, wherein said Fc variant further comprises an amino acid substitution at a position selected from the group consisting of 221, 222, 224, 227, 228, 230, 231, 223, 233, 234, 235, 236, 237, 238, 239, 240, 241, 243, 244, 245, 246, 247, 249, 250, 258, 262, 263, 264, 265, 266, 268, 269, 270, 271, 272, 273, 274, 275, 276, 278, 280, 281, 283, 285, 286, 288, 290, 291, 293, 294, 295, 296, 297, 298, 299, 300, 302, 313, 317, 318, 320, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 333, 334, 335 336 and 428, wherein numbering is according to the EU index, and wherein said Fc variant comprises an engineered glycoform.

A protein comprising an Fc variant of a parent Fc polypeptide, wherein said Fc variant comprises an amino acid modification at position 332, wherein said Fc variant has greater affinity for an Fc gamma receptor relative to said parent Fc polypeptide, wherein numbering is according to the EU index, and wherein said Fc variant comprises an engineered glycoform.