Method for silver staining of biological substances
AssigneeKyoto University
InventorAsako Okawa
Life of this patent
Estimating the term…
Abstract
A method for silver staining a gel on which biological substances such as nucleic acids and proteins are separated by electrophoresis, which comprises at least the following steps: (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate; (b) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of sodium acetate; and (c) the step of immersing the gel obtained in the step (b) in a mixture of ethanol and an aqueous solution of silver nitrate.
Claims7
A method for silver staining of a gel on which a biological substance is separated by electrophoresis, which comprises at least the following steps:
- (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate;
- (b) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of sodium acetate;
- and
- (c) the step of immersing the gel obtained in the step (b) in a mixture of ethanol and an aqueous solution of silver nitrate.
A method for silver staining a gel on which a biological substance is separated by electrophoresis, which comprises at least the following steps:
- (a) the step of immersing an untreated gel after the electrophoresis in an aqueous solution of sodium thiosulfate;
- (a-2) the step of immersing the gel obtained in the step (a) in a mixture of ethanol and an aqueous solution of acetic acid;
- (b′) the step of immersing the gel obtained in the aforementioned step (a-2) in a mixture of ethanol and an aqueous solution of sodium acetate;
- and
- (c′) the step of immersing the gel obtained in the step (b′) in a mixture of ethanol and an aqueous solution of silver nitrate without washing the gel with water.
Dependencies are inferred from the claim text.