# US 7,662,344 B2 · Locking pipette tip and mounting shaft > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,662,344 B2 - Document ID: US7662344B2 - Status: Granted - Kind: B2 - Application number: 11/934,381 - Filed: 2007-11-02 - Published: 2008-11-20 - Granted: 2010-02-16 - Earliest claimed: 2006-10-24 - Assignees: Viaflo Corporation - Inventors: Gregory Mathus; Terrence Kelly; Richard Cote - Web page: https://patentgrep.com/patent/US7662344B2 ## CPC - B01L 3/0217: {of the plunger pump type} - B01L 3/0275: {Interchangeable or disposable dispensing tips} - B01L 3/0279: {co-operating with positive ejection means} - B01L 2200/023: adapted for different sizes of tubes, tips or container - B01L 2200/025: Align devices or objects to ensure defined positions relative to each other - B01L 2200/0689: Sealing - B01L 2200/087: Ergonomic aspects - B01L 2300/027: Digital display, e.g. LCD, LED - Y10T 137/0318: Processes - Y10T 436/2575: Volumetric liquid transfer - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract The invention relates to a pipette tip mounting shaft configuration and a disposable pipette tip having a matching configuration. The mounting shaft includes a locking section located above a lower sealing section. The locking section has outwardly extending locking lobes circumferentially spaced around the mounting shaft and located above a stop member that separates the lower sealing section from the upper locking section. In certain embodiments, the diameter of the mounting shaft below the stop member is reduced in order to lessen insertion and ejection forces, which is particularly helpful for hand-held multi-channel pipettors. In these embodiments, the lower sealing section contains either a frustoconical sealing section, or an annular groove and a sealing ring, such as a flouroelastomeric O-ring seal. ## Claims ### Claim 1 A pipetting system comprising: a disposable pipette tip having: a barrel with a lower opening through which liquid is aspirated into the barrel and dispensed from the barrel, a collar having an upper opening for receiving a pipette mounting shaft, the lower end of the collar having a larger inside diameter than the inside diameter at the upper end of the barrel, and a circumferential shelf that connects the lower end of the collar to the upper end of the barrel; and a pipette mounting shaft including: a lower section including an annular groove with a sealing ring located in the annular groove, and an upper locking section, the locking section of the mounting shaft including a stop that engages the circumferential shelf of the pipette tip when the mounting shaft is fully inserted into the collar of the pipette tip, two or more outwardly extending lobes spaced circumferentially around the locking section of the mounting shaft and located above the stop on the mounting shaft for engaging an inside surface of the collar, and recessed relief portions in the upper locking section of the mounting shaft spanning circumferentially between the lobes and recessed relative to the lobes such that the collar distorts outwardly at the lobes and inwardly at the relief portions when the pipette tip is fully mounted on the mounting shaft; wherein the sealing ring engages an inside surface of the tip barrel at an upper end of the barrel when the mounting shaft is fully inserted into the pipette tip. ### Claim 2 (depends on 1) The pipetting system as recited in claim 1 wherein: the inside surface of the pipette tip barrel includes a circumferential stabilization ring extending inward from the inside surface of the barrel below the circumferential shelf; and the sealing ring on the lower section on the mounting shaft is located below the locking section on the mounting shaft and engages the inside surface of the tip barrel at a location above the circumferential stabilization ring when the mounting shaft is fully inserted into the pipette tip. ### Claim 3 (depends on 1) The pipetting system as recited in claim 1 wherein the sealing ring is an O-ring made of a fluoroelastomeric material. ### Claim 4 (depends on 1) The pipetting system as recited in claim 1 wherein the sealing ring is an O-ring and the upper edge of the groove in which the O-ring resides is no more than about 0.015 inches below the circumferential shelf on the pipette tip when the mounting shaft is fully inserted into the pipette tip. ### Claim 5 (depends on 4) The pipetting system as recited in claim 4 wherein the center line of the O-ring is no more than about 0.03 inches below the circumferential shelf along the inside surface of the barrel of the pipette tip when the mounting shaft is fully inserted into the pipette tip. ### Claim 6 (depends on 2) The pipetting system as recited in claim 2 wherein the diameter of the lower section on the mounting shaft below the groove for the sealing ring tapers to become smaller thereby avoiding an interference fit between the mounting shaft and the stabilization ring on the pipette tip. ### Claim 7 (depends on 1) The pipetting system as recited in claim 1 wherein each lobe includes an inclined ramp portion that facilitates distortion of the pipette tip collar when the mounting shaft is inserted into the pipette tip. ### Claim 8 (depends on 1) The pipetting system as recited in claim 1 wherein the mounting shaft has at least three lobes for engaging an inside surface of the collar. ### Claim 9 (depends on 1) A pipetting system as recited in claim 1 wherein the system includes multiple pipette mounting shafts, each in accordance with the limitations recited in claim 1 for the pipette mounting shaft. ### Claim 10 A pipetting system comprising: a disposable pipette having: a barrel with a lower opening through which liquid is aspirated into the barrel and dispensed from the barrel, a collar having an upper opening for receiving a pipette tip mounting shaft, the lower end of the collar having an inside diameter larger than the inside diameter of the upper end of the barrel, and a circumferential shelf that connects the lower end of the collar to the upper end of the barrel; and a pipette tip mounting shaft having: a locking section including a stop that engages a circumferential shelf of the pipette tip when the mounting shaft is fully inserted into the collar of the pipette tip, two or more outwardly extending lobes spaced circumferentially around the locking section of the mounting shaft and located above the stop on the mounting shaft for engaging an inside surface of the collar, and recessed relief portions in the upper locking section of the mounting shaft spanning circumferentially between the lobes and recessed relative to the lobes such that the collar distorts outwardly at the lobes and inwardly at the relief portions when the pipette tip is fully mounted to the mounting shaft; and an annular groove with a sealing ring located in the annular groove, wherein the sealing ring on the mounting shaft engages the inside surface of the pipette tip when the mounting shaft is fully inserted into the pipette tip. ### Claim 11 (depends on 10, 12) A pipetting system as recited in claim 10 wherein the system includes multiple pipette mounting shafts, each in accordance with the limitations recited in claim 12 for the pipette mounting shaft. ### Claim 12 (depends on 10) A pipetting system as recited in claim 10 wherein: the locking section of the mounting shaft is an upper locking section and the mounting shaft further comprises a lower section which includes the annular groove and the sealing ring; and the sealing ring of the mounting shaft engages the upper end of the barrel of the disposable pipette tip when the mounting shaft is fully inserted into the pipette tip. ### Claim 13 (depends on 1, 10) A pipetting system as recited in claim 1 or 10 wherein the inside surface of the collar includes a substantially circumferential locking element extending inward from the inside surface of the collar and wherein the two or more outwardly extending lobes on the mounting shaft engage the locking element on the inside surface of the collar when the pipette tip is fully mounted on the mounting shaft. ### Claim 14 A pipetting system comprising: a disposable pipette tip having: a barrel with a lower opening through which liquid is aspirated into the barrel and dispensed from the barrel, a circumferential sealing ring extending inward from the inside surface of the barrel, a collar having an upper opening that receives a lower end of a pipette mounting shaft, a lower end of the collar having a larger inside diameter than the inside diameter of the upper end of the barrel, and a circumferential shelf that connects the lower end of the collar to the upper end of the barrel; and a pipette mounting shaft including: an upper locking section, the locking section of the mounting shaft including a stop that engages the circumferential shelf of the pipette tip when the mounting shaft is fully inserted into the collar of the tip, two or more outwardly extending lobes spaced circumferentially around the locking section of the mounting shaft and located above the stop on the mounting shaft for engaging the inside surface of the collar, and recessed relief portions in the upper locking section of the mounting shaft spanning circumferentially between the lobes and recessed relative to the lobes such that the collar distorts outwardly at the lobes and inwardly at the relief portions when the pipette tip is fully mounted on the mounting shaft; and a lower section with a frustoconical portion located below the locking section, wherein said frustoconical portion area of the mounting shaft provides an interference fit with the circumferential sealing ring on the pipette tip when the mounting shaft is inserted into the pipette tip, the height of the frustoconical portion accommodating a range of vertical travel for interfering engagement of the circumferential sealing ring on the pipette tip against the tip mounting shaft before full engagement of the stop on the mounting shaft against the shelf of the pipette tip. ### Claim 15 (depends on 14) A pipetting system as recited in claim 14 wherein the inside diameter of the circumferential sealing ring on the pipette tip is at least the same or greater than the diameter of the mounting shaft below the frustoconical portion. ### Claim 16 (depends on 14) A pipetting system as recited in claim 14 wherein the frustoconical portion has an included angle of between about 4° and about 7° with respect to an imaginary cylindrical surface intersecting the frustoconical portion. ### Claim 17 (depends on 14) A pipetting system as recited in claim 14 wherein the inside surface of the collar includes a substantially circumferential locking element extending inward from an inside surface of the collar and the two or more outwardly extending lobes on the mounting shaft engage the locking element on the collar when the pipette tip is fully mounted on the pipette mounting shaft.