Method for making homozygous genetic modifications
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsWilliam Poueymirou, Thomas M. DeChiara, Wojtek Auerbach, David Frendewey, David M. Valenzuela
Life of this patent
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Abstract
Methods of generating modified embryos and mammals by introduction of donor cells into an early stage embryo are provided, such that the resulting embryo and animal generated therefrom has a significant contribution to all tissues from the donor cells and is capable of transmitting the donor cell DNA.
Claims18
A method of generating a mouse wherein at least 90% of the cells in said mouse comprise a homozygous genetic modification, the method comprising:
- (a) incorporating a donor mouse embryonic stem (ES) cell from an inbred strain into a pre-morula diploid mouse host embryo, wherein the donor ES cell is homozygous for the genetic modification;
- (b) culturing the pre-morula host embryo of (a) to the blastocyst stage;
- introducing the embryo of (b) into a surrogate mother for gestation, and
- (c) generating a mouse having at least a 90% cellular contribution from the donor ES cell, wherein at least 90% of the cells in said mouse are homozygous for the genetic modification.
A method of generating a mouse embryo wherein at least 90% of the cells in said mouse embryo comprise a homozygous genetic modification, the method comprising:
- (a) incorporating a donor mouse ES cell from an inbred strain into a pre-morula diploid mouse host embryo, wherein the donor ES cell is homozygous for the genetic modification;
- and
- (b) culturing the pre-morula host embryo of (a), wherein a mouse embryo comprising at least a 90% cellular contribution from the donor ES cell is generated and wherein at least 90% of the cells in said mouse embryo are homozygous for the genetic modification.
Dependencies are inferred from the claim text.