Human salivary gland-origin stem cell
AssigneeBios Research Institute Inc.
InventorsFumio Endo, Kenji Okumura, Kimitoshi Nakamura
Life of this patent
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Abstract
A novel human stem cell which can be differentiated to cells constituting a plurality of human organs including human liver is disclosed. The human stem cell according to the present invention is originated from human salivary gland, which is CD49f-positive, and which can be differentiated to (1) a nestin-positive and albumin-positive cell, (2) an insulin-positive cell and (3) a glucagon-positive cell by culture in vitro.
Claims19
A method for obtaining insulin-positive cells, comprising:
- culturing human salivary gland cells in vitro to obtain human stem cells which are nestin-positive and albumin-positive;
- differentiating said human stem cells which are nestin-positive and albumin-positive by culture in vitro to induce insulin-positive cells, and collecting the induced insulin-positive cells.
A method for obtaining glucagon-positive cells, comprising:
- culturing human salivary gland cells in vitro to obtain human stem cells which are nestin-positive and albumin-positive;
- differentiating said human stem cells which are nestin-positive and albumin-positive by culture in vitro to induce glucagon-positive cells, and collecting the induced glucagon-positive cells.
A method for obtaining insulin-positive cells, comprising:
- culturing CD49f-positive human salivary gland cells in vitro in the presence of epidermal growth factor to obtain human stem cells which are nestin-positive and albumin-positive;
- differentiating said human stem cells which are nestin-positive and albumin-positive by spheroid culture in vitro in the presence of epidermal growth factor, fibroblast growth factor and leukemia inhibitory factor to induce insulin-positive cells;
- and
- collecting the induced insulin-positive cells.
A method for obtaining glucagon-positive cells, comprising:
- culturing CD49f-positive human salivary gland cells in vitro in the presence of epidermal growth factor to obtain human stem cells which are nestin-positive and albumin-positive;
- differentiating said human stem cells which are nestin-positive and albumin-positive by spheroid culture in vitro in the presence of epidermal growth factor, fibroblast growth factor and leukemia inhibitory factor to induce glucagon-positive cells;
- and
- collecting the induced glucagon-positive cells.
Dependencies are inferred from the claim text.