Back to results
US 7,658,033 B2Granted
Method and apparatus for preparation of genetically transformable plant tissue
AssigneeMonsanto Technology LLC
InventorsBrian J. Martinell, Beth Jo Calabotta, Richard J. Heinzen, Richard F. Klemm, Dennis E. McCabe, Gail A. Roberts, Lori Ann Smith
Life of this patent
Estimating the term…
Abstract
A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high-throughput of viable, transplantable tissue.
Claims12
An apparatus for bulk preparation of transformable plant tissue comprising:
- (a) a first container with a sieve bottom for receiving plant seeds;
- (b) a second container sized to receive the first container therein;
- and
- (c) an agitator assembly comprising an air jet positioned in the second container beneath the first container, so that when the second container is filled with liquid, the agitator assembly may agitate the liquid around the seeds in the first container to divide the seeds into a separate cotyledon, seed coat and embryo.
1 more element
An apparatus for bulk preparation of transformable plant tissue comprising:
- (a) a first container with a sieve bottom for receiving plant seeds;
- (b) a second container sized to receive the first container therein;
- (c) an agitator assembly positioned in the second container beneath the first container, so that when the second container is filled with liquid, the agitator assembly may agitate the liquid around the seeds in the first container to divide the seeds into a separate cotyledon, seed coat;
- and embryo;
- and
- an agitator controller providing a series of pulses of the agitator to provide cycles of agitation and settling of the seeds.
3 more elements
Dependencies are inferred from the claim text.