siRNA targeting DNA-damage-inducible transcript 4 (DDIT4)
AssigneeDharmacon, Inc.
InventorsAnastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Steven Read, Stephen Scaringe
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Abstract
Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to DDIT4.
Claims20
An siRNA molecule effective at silencing protein expression, wherein said siRNA molecule comprises a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region comprising 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ. ID NO. 461.
An siRNA composition of between 19 and 30 base pairs in length that has 0-4 overhang regions, wherein said overhang regions are six or fewer bases in length, and wherein said composition comprises a sequence that is the complement of SEQ. ID. NO. 461.
A chemically synthesized double stranded siRNA molecule that directs cleavage of a target mRNA via RNA interference, wherein:
- a. each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length;
- and
- b. one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ. ID. NO. 461.
Dependencies are inferred from the claim text.