System and method for heating, cooling and heat cycling on microfluidic device

A microfluidic device for amplifying a nucleic acid comprising:

• a) a first plate with a first surface and a second surface;
• b) a first amplification chamber formed in said first surface;
• c) a microfluidic assay fluid circuit fluidly connected to said first amplification chamber;
• and
• d) a heat exchange film covering and sealing over said first amplification chamber, said heat exchange film with interior surface facing said first surface of said first plate and with oppositely facing exterior surface, wherein said interior surface of said heat exchange film is an optically transparent polyethylene terephthalate roll stock film, and wherein said heat-exchange film is joined to said first plate by a double sided ACA adhesive layer with laser cutout to seal around but not cover said amplification chambers.

A microfluidic device for amplifying a nucleic acid comprising:

• a) a first plate with a first surface and a second surface;
• b) a first amplification chamber formed in said first surface;
• c) a microfluidic assay fluid circuit fluidly connected to said first amplification chamber;
• and
• d) a heat exchange film covering and sealing over said first amplification chamber, said heat exchange film with interior surface facing said first surface of said first plate and with oppositely facing exterior surface, wherein said interior surface of said heat exchange film is an optically and UV transparent cyclic polyolefin roll stock film, and wherein said heat-exchange film is joined to said first plate by a double sided ACA adhesive layer with laser cutout to seal around but not cover said amplification chambers.

A microfluidic product for amplifying a nucleic acid, wherein

• a) said product comprises a first plate having at least one amplification chamber and a microfluidic assay circuit;
• b) said product is manufactured by a process comprising a step of coveringly joining an optically and UV transparent cyclic polyolefin heat exchange film to said first plate with a sandwich comprising a double-sided ACA layer of pressure sensitive adhesive, thereby sealingly enclosing said amplification chamber;
• and
• c) said ACA layer in said product is further characterized as having at least one laser cutout configured to expose the internal surface of the heat exchange film forming the cap of said amplification chamber.