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US 7,638,323 B2Granted
Fusion proteins and methods of producing same
AssigneeRecopharma AB
InventorsJan Holgersson, Jining Liu, Anki Gustafsson
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Abstract
The present invention provides compositions and methods for treating or preventing hyperacute rejection.
Claims13
A method of producing a mucin-immunoglobulin fusion polypeptide comprising:
- a) providing a cell comprising:
- i) a nucleic acid encoding a mucin polypeptide linked to a nucleic acid encoding at least a region of an immunoglobulin polypeptide;
- ii) a nucleic acid encoding an α1,3 galactosyltransferase polypeptide;
- and
- iii) a nucleic acid encoding a β 1,6,-N-acetylglucosaminyltransferase polypeptide;
- b) culturing the cell under conditions that permit production of said mucin-immunoglobulin fusion polypeptide wherein said fusion polypeptide is glycosylated by said α1,3 galactosyltransferase and said β 1,6,-N-acetylglucosaminyltransferase;
- and
- c) isolating said mucin-immunoglobulin fusion polypeptide.
5 more elements
A method of producing a mucin-immunoglobulin fusion polypeptide comprising:
- a) introducing into a cell
- i) a nucleic acid encoding a mucin polypeptide linked to a nucleic acid encoding at least a region of an immunoglobulin polypeptide,
- ii) a nucleic acid encoding an α1,3 galactosyltransferase polypeptide;
- and
- iii) a nucleic acid encoding a β 1,6,-N-acetylglucosaminyltransferase polypeptide;
- b) culturing the cell under conditions that permit production of said mucin-immunoglobulin fusion polypeptide wherein said fusion polypeptide is glycosylated by said α1,3 galactosyltransferase polypeptide and said β 1,6,-N-acetylglucosaminyltransferase;
- and
- c) isolating said mucin-immunoglobulin fusion polypeptide.
5 more elements
A cell comprising:
- a) a nucleic acid encoding a mucin polypeptide linked to a nucleic acid encoding at least a region of an immunoglobulin polypeptide,
- b) a nucleic acid encoding an α1,3 galactosyltransferase polypeptide;
- c) and a nucleic acid encoding a β 1,6,-N-acetylglucosaminyltransferase.
Dependencies are inferred from the claim text.