Gene encoding a nonribosomal peptide synthetase for the production of ramoplanin
AssigneeThallion Pharmaceuticals Inc.
InventorsChris M. Farnet, Emmanuel Zazopoulos, Alfredo Staffa
Life of this patent
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Abstract
The present invention relates to isolated genetic sequences encoding nonribosomal peptide synthetase (NRPS) proteins which direct the biosynthesis of the antibiotic ramoplanin in microorganisms such as Actinoplanes sp. The isolated gene sequences serve as substrates for bioengineering of antibiotic structures.
Claims25
An isolated nucleic acid comprising a polynucleotide which encodes a domain of a ramoplanin nonribosomal peptide synthetase, wherein said ramoplanin nonribosomal peptide synthetase comprises the amino acid sequence of SEQ ID NO:
- 14, and wherein said domain is condensation domain, an adenylation domain or a thiolation domain.
An isolated nucleic acid comprising a polynucleotide which encodes a module of a nonribosomal peptide synthetase comprising a domain of the ramoplanin nonribosomal peptide synthetase of SEQ ID NO:
- 14, wherein said module is selected from the group consisting of (i) module 1, consisting of amino acids 1-1059of SEQ ID NO:14, (ii) module 2, consisting of amino acids 1106-2122 of SEQ ID NO:14, (iii) module 3, consisting of amino acids 2159-3191 of SEQ ID NO:14, (iv) module 4, consisting of amino acids 3237-4228 of SEQ ID NO:14, (v) module 5, consisting of amino acids 4241-5260 of SEQ ID NO:14, (vi) module 6, consisting of amino acids 5307-5824 of SEQ ID NO:14, and (vii) module 7, consisting of amino acids 5838-6873 of SEQ ID NO:14.
An isolated polynucleotide molecule encoding a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.