# US 7,635,488 B2 · Patches and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 7,635,488 B2 - Document ID: US7635488B2 - Status: Granted - Kind: B2 - Application number: 11/411,531 - Filed: 2006-04-26 - Published: 2007-03-01 - Granted: 2009-12-22 - Earliest claimed: 2001-03-31 - Assignees: DBV Technologies - Inventors: Christophe Dupont; Bertrand Dupont; Pierre-Yves Vannerom; Pierre-Henri Benhamou; Jorge Ronco - Web page: https://patentgrep.com/patent/US7635488B2 ## CPC - A61B 10/0035: {Vaccination diagnosis other than by injuring the skin, e.g. allergy test patches} - A61K 49/0006: {Skin tests, e.g. intradermal testing, test strips, delayed hypersensitivity} - A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) - A61B 2017/00526: {Methods of manufacturing} - A61F 2013/0296: {for making transdermal patches (chemical processes excluded)} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A61F: FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS (dental prosthetics A61C) ## Abstract A patch comprising a support having electrostatic properties is provided wherein a periphery of the support is coated with an adhesive material, and all or part of the non-adhesive surface of the support is covered with at least one biologically active substance in the form of particles that is adhered to the non-adhesive part of the support by electrostatic forces. ## Claims ### Claim 1 A patch, comprising: a support defining non-overlapping first and second regions, the support having an electrical charge impressed upon the first region by application of an electrical field to form an electrically charged surface; a predetermined amount of a powdered biologically active substance bound to the electrically charged surface in the first region through electrostatic forces; and an adhesive disposed only in the second region, the adhesive configured to seal the patch against a patient's skin form a moisture-retaining chamber. ### Claim 2 (depends on 1) The patch of claim 1, wherein the powdered biologically active substance has an electrical charge opposite to the electrical charge of the electrically charged surface of the support. ### Claim 3 (depends on 1) The patch of claim 1, wherein the second region subdivides the first region into a plurality of strips. ### Claim 4 (depends on 1) The patch of claim 1, wherein the first region comprises a plurality of linear, circular, or elliptic strips and the second region comprises a plurality of linear, circular, or elliptic strips interposed between adjacent strips of the first region. ### Claim 5 (depends on 4) The patch of claim 4, wherein the support is rectangular. ### Claim 6 (depends on 1) The patch of claim 1, wherein the first region is circular and the second region circumscribes the first region. ### Claim 7 (depends on 6) The patch of claim 6, wherein the second region is annular. ### Claim 8 (depends on 7) The patch of claim 7, wherein the support is disk-shaped. ### Claim 9 (depends on 1) The patch of claim 1, wherein the support is moisture impermeable. ### Claim 10 (depends on 1) The patch of claim 1, further comprising a label removably coupled to the adhesive. ### Claim 11 (depends on 1) The patch of claim 1, wherein the biologically active substance is selected from an allergen, an antigen and a biologically active polypeptide. ### Claim 12 A method of manufacture of a skin patch, comprising: providing a support having a surface; applying an electrical field to the support to impress an electrical charge on the surface of the support to create an electrically charged surface; providing particles of a powdered active substance; and contacting the particles of the powdered active substance to the electrically charged surface so that the particles of the powdered active substance become bound to the electrically charged surface by electrostatic forces. ### Claim 13 (depends on 12) The method of claim 12, wherein impressing the electrical charge on the surface of the support comprises: selecting a support that can accumulate electrostatic charges; heating the support; applying an electric field to the support for a predetermined duration of time to electrically charge a surface of the support; cooling the film while continuing to apply the electric field to the support; and removing the electric field after the support is cooled. ### Claim 14 (depends on 13) The method of claim 13, wherein a polarity of the electric field applied to the support is selected to be opposite to a polarity of the powdered active substance. ### Claim 15 (depends on 12) The method of claim 12, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises: circulating the powdered active substance within a container; and exposing at least a portion of the electrically charged surface to the circulating particles of the powdered active substance. ### Claim 16 (depends on 12) The method of claim 12, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises blowing the particles of the powdered active substance toward the electrically charged surface with compressed air. ### Claim 17 (depends on 12) The method of claim 12, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises attracting the particles of the powdered active substance toward the electrically charged using an electric field. ### Claim 18 (depends on 12) The method of claim 12, wherein providing the particles of the powdered active substance comprises: creating a solid form of an active substance by lyophilization or heating and spraying; and grinding the solid form to create solid particles having a predetermined and controlled size. ### Claim 19 (depends on 1) A method for testing the sensitization state of a subject with respect to an allergen, the method comprising applying the patch of claim 1 to the skin of the subject, wherein said biologically active substance is the allergen, and detecting the presence or absence of a skin reaction to the allergen. ### Claim 20 (depends on 1) A method for stimulating an immune response to an antigen in a subject, the method comprising applying the patch of claim 1 to the skin of the subject, wherein said biologically active substance is the antigen or an epitope thereof. ### Claim 21 (depends on 20) The method of claim 20, wherein the antigen is a polypeptide or a protein. ### Claim 22 (depends on 1) A method for delivering a drug to a subject, the method comprising a patch of claim 1 to the skin of the subject, wherein said biologically active substance is the drug. ### Claim 23 (depends on 22) The method of claim 22, wherein the drug is a polypeptide or a protein. ### Claim 24 A patch, comprising: a support having non-overlapping first and second portions, the first portion comprising a material capable of accumulating an electrostatic charge, the first portion having an electrically charged surface created by applying an electrical field to the first portion to impress an electrical charge thereon; a layer of powdered active particles adhered to the first portion by contacting particles of a powdered active substance to the electrically charged surface; and an adhesive disposed on the second portion, the adhesive configured to adhere the patch to skin. ### Claim 25 (depends on 24) The patch of claim 24, wherein the electrical charge is impressed onto the first region by heating the support, applying the electric field for a predetermined duration of time, cooling the support while continuing to apply the electric field, and then removing the electric field after the support has cooled. ### Claim 26 (depends on 24) The patch of claim 24, wherein the particles of powdered active substance have an electrical charge opposite to an electrical charge of the electrically charged surface. ### Claim 27 (depends on 24) The patch of claim 24, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises: circulating the particles of powdered active substance within a container; and exposing at least a portion of the electrically charged surface to the circulating particles of the powdered active substance. ### Claim 28 (depends on 24) The patch of claim 24, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises blowing the particles of the powdered active substance toward the electrically charged surface with compressed air. ### Claim 29 (depends on 24) The patch of claim 24, wherein contacting the particles of the powdered active substance to the electrically charged surface comprises attracting the particles of the powdered active substance toward the electrically charged using an electric field. ### Claim 30 (depends on 24) The patch of claim 24, wherein providing the particles of the powdered active substance comprises creating a solid form of an active substance by lyophilization or heating and spraying and then grinding the solid form to create solid particles having a predetermined and controlled size. ### Claim 31 (depends on 24) The patch of claim 24, wherein the second region subdivides the first region into a plurality of strips. ### Claim 32 (depends on 31) The patch of claim 31, wherein the first region comprises a plurality of linear, circular, or elliptic strips and the second region comprises a plurality of linear, circular, or elliptic strips interposed between adjacent strips of the first region. ### Claim 33 (depends on 24) The patch of claim 24, wherein the support is rectangular. ### Claim 34 (depends on 24) The patch of claim 24, wherein the first region is circular and the second region circumscribes the first region. ### Claim 35 (depends on 34) The patch of claim 34, wherein the second region is annular. ### Claim 36 (depends on 24) The patch of claim 24, wherein the support is moisture impermeable. ### Claim 37 (depends on 24) The patch of claim 24, further comprising a label removably coupled to the adhesive. ### Claim 38 (depends on 24) The patch of claim 24, wherein the powdered active substance is selected from an allergen, an antigen and a biologically active polypeptide. ### Claim 39 (depends on 1) The patch of claim 1 wherein the support comprises glass or a polymer. ### Claim 40 (depends on 39) The patch of claim 39, wherein the support is selected from amongst the group consisting of: glass, cellulose plastics, polyethylene, polyester, polyvinyl chlorides, polypropylenes, polystyrenes, polycarbonates, polyacrylics, and fluoropolymers. ### Claim 41 (depends on 24) The patch of claim 24 wherein the support comprises glass or a polymer. ### Claim 42 (depends on 41) The patch of claim 41, wherein the support is selected from amongst the group consisting of: glass, cellulose plastics, polyethylene, polyester, polyvinyl chlorides, polypropylenes, polystyrenes, polycarbonates, polyacrylics, and fluoropolymers.