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US 7,632,663 B1Granted
Method for microbially producing L-valine
AssigneeForschungszentrum Julich GmbH
InventorsLothar Eggeling, Hermann Sahm
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AssigneeForschungszentrum Julich GmbH
InventorsLothar Eggeling, Hermann Sahm
Estimating the term…
The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.
A microorganism transformed with a nucleotide sequence encoding dihydroxy acid dehydratase (ilvD), nucleotide sequences encoding acetohydroxy acid synthase and isomeroreductase (ilvBNC) or both ilvD and ilvBNC, in which microorganism the activity of one or more enzymes that are specifically involved in the synthesis of D-pantothenate is reduced or eliminated,
A method for the production of L-valine comprising the step of culturing a microorganism transformed with a nucleotide sequence encoding dihydroxy acid dehydratase (ilvD) and nucleotide sequences encoding acetohydroxy acid synthase and isomeroreductase (ilvBNC), under conditions wherein said microorganism produces L-valine, wherein said microorganism is a Corynebacterium species and said nucleotide sequence encoding ilvD comprises the portion of SEQ ID NO:
A method for the production of L-valine comprising the step of culturing a microorganism in which the activity of dihydroxy acid dehydratase (ilvD) is increased by increased expression of the ilvD nucleotide sequence encoding ilvD, under conditions wherein said microorganism produces L-valine, wherein said microorganism is a Corynebacterium species and said nucleotide sequence encoding ilvD comprises the portion of SEQ ID NO:
Dependencies are inferred from the claim text.