Transformed cell harboring DNA or RNA encoding sugar chain synthesizing agent having β1,3-N-acetylglucosaminyltransferase activity

A method for producing a cell having increased amount of a poly-N-acetyllactosamine sugar chain, a lacto-series glycolipid (a glycolipid having Galβ1-3GlcNAcβ1-3Galβ1-4Glc-ceramide as a backbone) or a neolacto-series glycolipid (a glycolipid having Galβ1-4GlcNAcβ1-3Galβ1-4-ceramide as a backbone), which comprises the steps of:

• introducing DNA or RNA into a cell to express a polypeptide encoded by the DNA or RNA, wherein said DNA or RNA is selected from the group consisting of the following (a) to (g):
• (a) a DNA encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:1 and having a beta 1,3-N-acetylglucosaminyltransferase activity,
• (b) a DNA encoding a polypeptide comprising an amino acid sequence of positions 39-378 in the amino acid sequence of SEQ ID NO:1 and having a beta 1,3-N-acetylglucosaminyltransferase activity,
• (c) a DNA encoding a polypeptide comprising an amino acid sequence having 95% or more homology with the amino acid sequence in the polypeptide according to (a) or (b), and having a beta 1,3-N-acetylglucosaminyltransferase activity,
• (d) a DNA comprising the nucleotide sequence of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity,
• (e) a DNA comprising the nucleotide sequence of positions 135 to 1268 of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity,
• (f) a DNA comprising the nucleotide sequence of positions 249 to 1268 of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity, and
• (g) an RNA comprising a sequence corresponding to the DNA selected from any one of (a) to (g);
• and
• culturing the cells in order to express said polypeptide.