Linking of images to enable simultaneous viewing of multiple objects

A computerized method for linking digitized images of serial sections of a biological tissue sample such that the digitized images of the serial sections are displayed on a computer display that is controlled by a computer, the method comprising:

• creating a copy of a digitized image of a first serial section, the copy being referred to as the ghost image, wherein the digitized image of the first serial section includes at least a first set of image data and a second set of image data that have different image-density information;
• positioning the ghost image over a digitized image of a second serial section, the digitized image of the second serial section being visible through the ghost image and being referred to as the underlying image;
• changing the transparency of the ghost image to effect change of the visualization of the underlying image;
• registering the ghost image to the underlying image;
• linking the ghost image and the underlying image such that when manipulations are performed on either the ghost image or the underlying image, the other of the images is similarly manipulated;
• scanning the first serial section at a first magnification to generate the first set of image data;
• and
• scanning the first serial section at a second magnification to generate the second set of image data,
• wherein the first magnification is greater than the second magnification, and
• wherein a data density of the first set of image data is greater than a density of the second set of image data.